Name     

Affiliation     

Country     

E-mail     

Application area     

Please tell us how you learned about FCS-Bayes

END-USER LICENSE AGREEMENT

BY CLICKING ON THE "ACCEPT" BUTTON AT THE END OF THIS AGREEMENT, YOU ARE CONSENTING TO BE BOUND BY ALL OF THE TERMS OF THIS AGREEMENT. IF THE "ACCEPT" BUTTON IS NOT PRESENT OR OTHERWISE DISABLED, INSTALLING AND USING THE SOFTWARE ACCOMPANYING THIS LICENSE INDICATES YOUR ACCEPTANCE OF THESE TERMS AND CONDITIONS. IF YOU DO NOT AGREE TO ALL THE TERMS AND CONDITIONS OF THIS AGREEMENT, CLICK THE "DO NOT ACCEPT" BUTTON, AND THE INSTALLATION PROCESS WILL NOT CONTINUE.

MASSACHUSETTS INSTITUTE OF TECHNOLOGY

END-USER LICENSE AGREEMENT FOR RESEARCH SOFTWARE

Redistribution Not Permitted

© 2012 Massachusetts Institute of Technology. All rights reserved.

THIS SOFTWARE IS BEING LICENSED FREE OF CHARGE FOR RESEARCH AND NON-COMMERCIAL PURPOSES ONLY

The Massachusetts Institute of Technology ("MIT"), a non-profit institution of higher education, agrees to make the downloadable software and documentation, if any, (collectively, the "Software") available to you without charge for demonstrational and non-commercial purposes, subject to the following terms and conditions.

1. Internal Use License. MIT hereby grants to you a non-transferable, nonexclusive royalty- free license (without any right to sublicense or distribute) to use the Software in machine- readable, object code form only and only as authorized in this License Agreement. You may use the Software on any single computer; use the Software on a second computer so long as the primary user of each copy is the same person and more than one copy is not simultaneously used; or copy the Software for archival purposes, provided any copy must contain all of the proprietary notices on the original Software. Neither concurrent use on two or more computers nor use in a local area network or other network is permitted, except as required by the game to function in a multiplayer environment provided that all users have their own separate authorization.

2. Restrictions. You may not modify, translate, reverse engineer, decompile, disassemble, or create derivative works based on the Software. You may not copy the Software, except as specified above. You may not sublicense, rent, lease, or otherwise transfer rights to the Software. You may not remove any proprietary notices or labels on the Software.

3. No Other Rights. MIT claims and reserves title to the Software and all rights and benefits afforded under any available United States and international laws protecting copyright and other intellectual property rights in the Software. Nothing herein shall be construed to limit the rights of MIT and its licensees to use, modify, adapt, or enhance the Software, or any derivative works based on the Software, for their own purposes. Nothing herein shall be construed to expand your rights with respect to the Software beyond those rights expressly granted you in paragraph 1.

4. Termination. This License Agreement is effective upon your expressing agreement by clicking the "Agree" button below, or for instances when no button is present or otherwise disabled, when installing and using the software accompanying this license. This license will terminate automatically if you fail to comply with the limitations described above. On termination, you must uninstall and/or destroy all copies of the Software.

5. Export Controls. You acknowledge that export of the Software may be subject to compliance with various laws, rules, and regulations that restrict the export of certain products and technical data. If you are within the United States, you represent that you will not export the Software outside of the United States, either directly or indirectly. If you are outside the United States, and if the Software is subject to U.S. export control restrictions, you are only allowed to obtain the Software in strict compliance with any applicable restrictions. If these restrictions are not observed, this license shall be null and void, and you shall immediately return or destroy the Software and all copies.

6. Disclaimer of Warranty. You accept the Software on an "AS IS" basis. MIT MAKES NO REPRESENTATIONS OR WARRANTIES CONCERNING THE SOFTWARE, AND EXPRESSLY DISCLAIMS ALL SUCH WARRANTIES, INCLUDING WITHOUT LIMITATION ANY EXPRESS OR IMPLIED WARRANTY OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, AND NON-INFRINGEMENT OF INTELLECTUAL PROPERTY RIGHTS. MIT has no obligation to assist in your installation or use of the Software or to provide services or maintenance of any type with respect to the Software. The entire risk as to the quality and performance of the Software is borne by you. You acknowledge that the Software may contain errors or bugs. You must determine whether the Software sufficiently meets your requirements. This disclaimer of warranty constitutes an essential part of this Agreement.

7. No Consequential Damages; Indemnification. IN NO EVENT SHALL MIT BE LIABLE TO YOU FOR ANY LOST PROFITS OR OTHER INCIDENTAL OR CONSEQUENTIAL DAMAGES RELATING TO THE SUBJECT MATTER OF THIS AGREEMENT. To the extent permitted by applicable law, you agree to indemnify, defend, and hold harmless MIT and its directors, officers, representatives, employees, and agents against all losses, expenses (including without limitation any legal expenses), claims, demands, suits, or other actions arising from your use of the Software, except to the extent caused by the gross negligence or willful misconduct of MIT.

8. Miscellaneous. If any provision of this Agreement is held to be unenforceable for any reason, such provision shall be modified only to the extent necessary to make it enforceable. You may not assign this Agreement. This Agreement shall be governed by and construed in accordance with the laws of the Commonwealth of Massachusetts irrespective of any conflicts of law principles. You agree that any legal action arising out of or in connection with this Agreement shall be brought in the state or Federal courts, as appropriate, in Suffolk County, Massachusetts. The applicability of the United Nations Convention of Contracts for the International Sale of Goods is expressly excluded.

ACCEPT DO NOT ACCEPT


 

Fluorescence Correlation Spectroscopy (FCS) is a powerful approach to measuring biomolecular dynamics in living and reconstituted systems. Inferring the physical process underlying measured fluorescence fluctuations, including species concentrations, mode of transport, and binding reactions, requires the objective, side-by-side evaluation of multiple competing hypotheses based on FCS data. Bayesian inference provides a practical framework for this multiple hypothesis testing to be performed in an unbiased, automated manner accounting for sampling noise and model complexity. This approach is particularly useful for the analysis of novel biological and chemical systems for which the underlying physical process is unknown a priori, and imaging FCS data which typically contains hundreds to thousands of correlation functions in a single measurement. Our proposed approach operates directly either on raw fluorescence intensity traces or temporal autocorrelation functions. A Bayesian procedure for multiple hypothesis testing of FCS data is available here for download as a set of MATLAB resource files. Please visit MSD-Bayes for a related approach to analyzing single-particle motion based on single-particle tracking data.

 

The FCS-Bayes package has been downloaded 166 times